BPDST: Blockchain-Based Privacy-Preserving Data Sharing on Thin Client for Electronic Medical Records

Sharing medical data can improve the quality of medical services and reduce costs. However, the current Electronic Medical Records (EMRs) are scattered and easily tampered with, which is not conducive to the sharing of EMRs and is not compatible with thin clients. Fortunately, blockchain technology is tamper-proof, decentralized, auditable, and meets the above requirements. To solve these problems, we first propose Blockchain-Based Privacy-Preserving Data Sharing on Thin-Client for Electronic Medical Records (BPDST) approach that combines the k-anonymity and cloud storage, which thin clients can run like a full-node user and safeguard user\u27s EMRs privacy concurrently. Using this approach, patients can control their own EMRs, while the consortium blockchain is responsible for the transaction process and sending the correct results to the patients. BPDST can also share information without leaking or tampering with EMRs\u27 privacy, achieving the purpose of sharing medical data and privacy protection. In the medical field, this study can effectively protect users\u27 privacy when sharing medical data to provide convenience for users and break the "island" phenomenon among various medical institutions. Security analysis and extensive experiments show that BPDST is secure and practical for sharing EMRs

Intelligent Agents for Negotiation and Recommendation in Mass Customization

Mass customization, as a means to meet individual consumer’s need on a large scale, has recently attracted the attention of both researchers and practitioners. However, as customers and their needs grow increasingly diverse, meeting every consumer’s need has become a surefire way to add unnecessary cost and complexity to operations. Furthermore, consumers are not all really ready for mass customization. They have to face inconveniences such as expensive price, delay delivery and they have to spend time “designing” their product. In order to solve this problem, we proposed a way of intelligent agent assisted negotiation and recommendation. The recommendation is a preference elicitation process, while the negotiation is a communication process based on the situation of manufacturer, such as the inventory level, production cost and lead time. With the aid of intelligent agent of negotiation and recommendation, a good balance between efficiency and customer satisfactions of mass customization can be reached

Pelatihan Manajemen Bank Sampah Guna Pelestarian Lingkungan dan Meningkatkan Nilai Ekonomis Masyarakat Di Kecamatan Namorambe Kabupaten Deliserdang

AbstractAlong with the incrase population density especially in Deli Serdang, it can’t be separated from the incrase amount of waste.  The garbage will incrase with population too, this isi so importance to solve. The result from Environmental Service of Deli Serdang there was 1.078 tons/day the waste production in Deli Serdang. Most of the waste produced from household and the rest comes from companies, offices, school, and others. To solved this problems it is necessary socialitation to public for managing their waste.  If garbage managed properly it will be have a positive impact through composting and economic value added from a waste bank. The schools are the place that having the important role of social values to students, so they can think that they have to get knowledge about wasting management. From This PKM we expected the schools can get the knowledge about waste management methods that will be useful in society. This PKM activity is  a workshop to teachers and students especially about waste management. The output of the implementation from this activity is all of society in the school getting the knowledge and skill in waste management

BUANG AIR BESAR DI KEBUN (Studi Kasus pada Masyarakat Desa Pagar Gading, Kecamatan Pino Raya, Kabupaten Bengkulu Selatan)

Beberapa masyarakat di Desa Pagar Gading masih melakukan buang air besar di kebun. Padahal di desa ini sudah tersedia fasilitas jamban umum. Tujuan dari penelitian ini untuk mendeskripsikan dan mengetahui makna dan faktor penyebab buang air besar di kebun pada masyarakat Desa Pagar Gading. Penelitian ini menggunakan pendekatan deskriptif kualitatif. Penentuan informan menggunakan teknik snowball sampling. Pengumpulan data menggunakan teknik observasi, wawancara dan dokumentasi. Hasil penelitian menjelaskan bahwa masyarakat yang melakukan buang air besar di kebun dimaknai sebagai perilaku yang masih benar dilakukan sampai saat ini, perilaku buang air besar dikebun dianggap sebagai hal yang biasa, buang air besar dikebun dinilai lebih ekonomis, lebih praktis dan tidak menimbulkan masalah kesehatan. Hal ini disebabkan oleh: Faktor nilai-nilai kebenaran yang masih diyakini dan dipertahankan sampai saat ini sehinga masyarakat melakukan pembenaran atas perilaku buang air besar di kebun. Faktor kebiasaan buang air besar yang telah berlangsung lama, sejak dahulu secara turun temurun, sehingga menimbulkan persepsi bahwa perilaku buang air besar di kebun dianggap sebagai hal yang biasa. Tidak adanya sanksi dan larangan membuat masyarakat menganggap buang air besar di kebun tidak mengganggu orang lain. Faktor ekonomi masyarakat yang hanya mampu mencukupi kebutuhan pokok, sehingga masyarakat menilai buang air besar di kebun lebih ekonomis, tidak memerlukan biaya pembuatan tempat buang air besar. Rendahnya pengetahuan masyarakat sehingga sulit menerima informasi terkait buang air besar di kebun melalui berbagai media informasi. Serta minimnya sosialisasi terkait pemanfaatan jamban berdampak terhadap rendahnya pengetahuan masyarakat terkait dampak yang ditimbulkan dari perilaku buang air besar di kebun. Kata Kunci: Buang Air Besar di Kebu

A Review of the Engineering Role of Burrowing Animals: Implication of Chinese Pangolin as an Ecosystem Engineer

Ecosystem engineers are organisms that alter the distribution of resources in the environment by creating, modifying, maintaining and/or destroying the habitat. They can affect the structure and function of the whole ecosystem furthermore. Burrowing engineers are an important group in ecosystem engineers as they play a critical role in soil translocation and habitat creation in various types of environment.However, few researchers have systematically summarized and analyzed the studies of burrowing engineers. We reviewing the existing ecological studies of burrowing engineer about their interaction with habitat through five directions: (1) soil turnover; (2)changing soil physicochemical properties; (3) changing plant community structure; (4) providing limited resources for commensal animals;and/or (5) affecting animal communities. The Chinese pangolin (Manis pentadactyla) is a typical example of burrowing mammals, in part (5), we focus on the interspecific relationships among burrow commensal species of Chinese pangolin. The engineering effects vary with environmental gradient, literature indicates that burrowing engineer play a stronger role in habitat transformation in the tropical and subtropical areas.The most common experiment method is comparative measurements (include different spatial and temporal scale),manipulative experiment is relatively few. We found that most of the engineering effects had positive feedback to the local ecosystem, increased plant abundance and resilience, increased biodiversity and consequently improved ecosystem functioning. With the global background of dramatic climate change and biodiversity loss in recent decades, we recommend future studies should improving knowledge of long-term engineering effects on population scale and landscape scale, exploring ecological cascades through trophic and engineering pathways, to better understand the attribute of the burrowing behavior of engineers to restore ecosystems and habitat creation. The review is presented as an aid to systematically expound the engineering effect of burrowing animals in the ecosystem, and provided new ideas and advice for planning and implementing conservation management

Research hotspots and new trends in the impact of resistance training on aging, bibliometric and visual analysis based on CiteSpace and VOSviewer

PurposeResistance training (RT) can intervene in aging, which can effectively improve trainees’ life. However, unhealthy living habits such as irregular life, obesity and hyperlipidemia, and chronic diseases lead to a significant decline in the energy level of the population, seriously affecting the health of the population. Our research identifies the research hotspots of RT to intervene in aging from the perspective of bibliometrics, predicts research frontiers and development trends, and provides more perspectives for research on aging populations.MethodsIn this study, we used CiteSpace and VOSviewer visualization software to draw the scientific knowledge map of countries/regions, institutions, authors, co-occurrence keywords, and co-cited references of published articles, and explore the Web of Science core collection database all about the RT intervention aging research status, hotspots, frontiers, and development trends of articles on aging.ResultsAmong the 760 articles that meet the inclusion criteria, the number of articles published and the frequency of citations have increased steadily in the past 5 years. Judging from the countries/regions, institutions, scholars, and journals that published articles, the ones with the largest numbers are the USA, Univ Estadual Londrina, Cyrino ES, and Exp Gerontol. The ones with the highest influence are England, Univ Arkansas Med Sci, Frontera WR, and Biochem Biophys Rep Co. The top five co-occurrence keywords of include exercise, strength, resistance training, skeletal muscle, and muscle strength. The research frontier is physical function.ConclusionIn the field of RT intervention aging research, relevant scholars deserve further in-depth research and exploration. The United States, Brazil, Canada, and other economically developed countries/regions, institutions, and authors have greater influence and productivity. These quantitative research results can provide references for relevant scholars’ follow-up research and government departments to formulate and modify health policies or measures

A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit

Abstract Here we report the rescue of a recombinant porcine reproductive and respiratory syndrome virus (PRRSV) carrying an enhanced green fluorescent protein (EGFP) reporter gene as a separate transcription unit. A copy of the transcription regulatory sequence for ORF6 (TRS6) was inserted between the N protein and 3′-UTR to drive the transcription of the EGFP gene and yield a general purpose expression vector. Successful recovery of PRRSV was obtained using an RNA polymerase II promoter to drive transcription of the full-length virus genome, which was assembled in a bacterial artificial chromosome (BAC). The recombinant virus showed growth replication characteristics similar to those of the wild-type virus in the infected cells. In addition, the recombinant virus stably expressed EGFP for at least 10 passages. EGFP expression was detected at approximately 10 h post infection by live-cell imaging to follow the virus spread in real time and the infection of neighbouring cells occurred predominantly through cell-to-cell-contact. Finally, the recombinant virus generated was found to be an excellent tool for neutralising antibodies and antiviral compound screening. The newly established reverse genetics system for PRRSV could be a useful tool not only to monitor virus spread and screen for neutralising antibodies and antiviral compounds, but also for fundamental research on the biology of the virus.This study was funded by grants from the National Natural Science Foundation of China (U0931003/L01) and the National High-Tech R&D Program of China (2011AA10A208) to EMZ, the National Natural Science Foundation of China (31302103) to WCB, the European Community’s Seventh Frame-work Programme (PoRRSCon, FP7-KBBE-2009-3-245141) and the Ministry of Science and Innovation of Spain (MCINN) (BIO2010-16075) to FA and LE.Peer Reviewe

MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection

Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease

Characterization of DNA Methylation Associated Gene Regulatory Networks During Stomach Cancer Progression

DNA methylation plays a critical role in tumorigenesis through regulating oncogene activation and tumor suppressor gene silencing. Although extensively analyzed, the implication of DNA methylation in gene regulatory network is less characterized. To address this issue, in this study we performed an integrative analysis on the alteration of DNA methylation patterns and the dynamics of gene regulatory network topology across distinct stages of stomach cancer. We found the global DNA methylation patterns in different stages are generally conserved, whereas some significantly differentially methylated genes were exclusively observed in the early stage of stomach cancer. Integrative analysis of DNA methylation and network topology alteration yielded several genes which have been reported to be involved in the progression of stomach cancer, such as IGF2, ERBB2, GSTP1, MYH11, TMEM59, and SST. Finally, we demonstrated that inhibition of SST promotes cell proliferation, suggesting that DNA methylation-associated SST suppression possibly contributes to the gastric cancer progression. Taken together, our study suggests the DNA methylation-associated regulatory network analysis could be used for identifying cancer-related genes. This strategy can facilitate the understanding of gene regulatory network in cancer biology and provide a new insight into the study of DNA methylation at system level

Chicken Organic Anion-Transporting Polypeptide 1A2, a Novel Avian Hepatitis E Virus (HEV) ORF2-Interacting Protein, Is Involved in Avian HEV Infection

Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease in chickens. Due to the absence of a highly effective cell culture system, there are few reports about the interaction between avian HEV and host cells. In this study, organic anion-transporting polypeptide 1A2 (OATP1A2) from chicken liver cells was identified to interact with ap237, a truncated avian HEV capsid protein spanning amino acids 313 to 549, by a glutathione S-transferase (GST) pulldown assay. GST pulldown and indirect enzyme-linked immunosorbent assays (ELISAs) further confirmed that the extracellular domain of OATP1A2 directly binds with ap237. The expression levels of OATP1A2 in host cells are positively correlated with the amounts of ap237 attachment and virus infection. The distribution of OATP1A2 in different tissues is consistent with avian HEV infection in vivo. Finally, when the functions of OATP1A2 in cells are inhibited by its substrates or an inhibitor or blocked by ap237 or anti-OATP1A2 sera, attachment to and infection of host cells by avian HEV are significantly reduced. Collectively, these results displayed for the first time that OATP1A2 interacts with the avian HEV capsid protein and can influence viral infection in host cells. The present study provides new insight to understand the process of avian HEV infection of host cells